One-step purification/extraction method to access glyphosate, glufosinate, and their metabolites in natural waters

A new green method for trace level quantification of four herbicides, glyphosate (GLYP), glufosinate (GLUF), and their main metabolites, aminomethylphosphonic acid (AMPA) 3-(methyl-phosphinico)-propionic (MPPA), was developed. The purification step without any derivatization conducted by solid-phase extraction using Chelex-100 resin in the Fe (III) form, followed elution with 5% NH4OH. analytes were quantified ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. developed validated on five fresh sea water matrices mean recoveries ranging from 80.1% 109.4% (relative standard deviation < 20%). conditions evaluated certified high applicability too. limits detection (ng/L) ranges 0.70 – 4.0, 2.4 3.9, 1.8 4.7, 1.6 4.0 GLYP, AMPA, GLUF, MPPA, respectively. successfully applied detect compounds surface waters sampled along Red River Delta region July 2019. highest concentrations detected at 565, 1,330, 234, 871 ng/L These results showed potential capacity this convenient monitoring herbicides metabolites diverse natural system.